Unicycler

A hybrid assembly pipeline for bacterial genomes

Unicycler

Note

This pipeline uses SPAdes

Unicycler Long Reads

Parameters

  • FASTQ file: file

    Sequencing raw read file. Can be short or long read

Returns

_images/unicyclerLong.png

  1. Genome Assembly: Tab-separated annotation

    Assembly FASTA File

_images/assemblyLongUnicycler.png

  1. GFA File: File

    Can be used in 3rd party tools like Bandage

Unicycler Short Reads

Parameters

  • FASTQ first file: file

    First Read file (paired or single-end)

  • FASTQ second file: file

    Second read file (paired only)

Returns

_images/unicyclerShort.png

  1. Genome Assembly: Tab-separated annotation

    Assembly FASTA File

  2. GFA File: File

    Can be used in 3rd party tools like Bandage